mouse monoclonal antibodies against gad65  (Developmental Studies Hybridoma Bank)

Journal: Neuroscience

Article Title: IMMUNOCYTOCHEMICAL IDENTIFICATION OF ELECTRONEUTRAL NA + -COUPLED HCO 3 − TRANSPORTERS IN FRESHLY DISSOCIATED MOUSE MEDULLARY RAPHÉ NEURONS

doi: 10.1016/j.neuroscience.2013.02.064

Figure Lengend Snippet: Identification of neuronal cell types in the MR, using mouse monoclonal antibodies. (A) The IgG1 monoclonal MAP2 antibody, together with the Cyanine-2-tagged anti-IgG1 secondary antibody (green), identifies a neuron (upper left panel) that is also shown in a DIC image (upper right). In the absence of the primary antibody, the secondary antibody did not recognize any cells, one example of which is shown here as a negative control (lower left). The DIC image shows the cell at this location (lower right). (B) Representative z-sections for neuron on left side of panel A (details below). (C) The IgG3 monoclonal TPH antibody, together with the Cyanine-5-tagged anti-IgG3 secondary antibody (magenta), identifies a serotonergic neuron (upper left panel). The staining of the secondary antibody is virtually eliminated in the absence of the primary antibody (lower left panel). The two right panels show the DIC images. (D) Representative z-sections for neuron on left side of panel C (details below). (E) IgG3 monoclonal GAD2 antibody, together with the Cyanine-5-tagged anti-IgG3 secondary antibody (cyan), identifies a GABAergic neuron (upper left panel). The staining of the secondary antibody is virtually eliminated in the absence of the primary antibody (lower left panel). The two right panels show the DIC images. Details on z-sections (panels B, D, F): For each, the main picture is an xy section (MAP2 500 nm/slice; TPH 60 nm/slice; GAD2 235 nm/slice) per slice. The yellow cross-hairs indicate the planes of the two side views: the xz section (shown below the main picture) and the yz section (shown to the right of the main picture). In the xz and yz sections, the green lines show the plane of the xy section. Scale bar = 10 μm. Images were acquired with a 60× objective. These panels show that the MAP2, TPH, and GAD2 antibodies are within the cytoplasm but excluded from the nucleus. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)

Article Snippet: An anti-GAD2 antibody (mouse monoclonal IgG3, clone 26H1, directed against the GAD65, the 65 kDa isoform) was purchased from SySy, Goettingen, Germany.

Techniques: Negative Control, Staining

Journal: Neuroscience

Article Title: IMMUNOCYTOCHEMICAL IDENTIFICATION OF ELECTRONEUTRAL NA + -COUPLED HCO 3 − TRANSPORTERS IN FRESHLY DISSOCIATED MOUSE MEDULLARY RAPHÉ NEURONS

doi: 10.1016/j.neuroscience.2013.02.064

Figure Lengend Snippet: Frequency distributions of the individual cell types. The data summarized here come from many images like that in Fig. 1. (A) The mean frequency distribution (green histogram) of 10 MAP2-positive cells describes the average number of pixels for each pixel intensity. The mean intensity of cells in the MAP2 population was 876 (arbitrary units, a.u.). (B) The frequency distribution of the negative controls (black histogram) is the average of 10 cells. The average mean intensity from negative controls was 177 (a.u.). The green data represent a re-plot of the data from panel A. (C) The mean frequency distribution (Cyan histogram) of 13 GAD2-positive cells yields a mean intensity of 1175 (a.u.). (D) The frequency distribution of the negative controls (black histogram) is an average of 20 cells. The average mean intensity from negative controls was 220 (a.u.). The cyan data represent a re-plot of the data from panel C. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)

Article Snippet: An anti-GAD2 antibody (mouse monoclonal IgG3, clone 26H1, directed against the GAD65, the 65 kDa isoform) was purchased from SySy, Goettingen, Germany.

Techniques:

Journal: Neuroscience

Article Title: IMMUNOCYTOCHEMICAL IDENTIFICATION OF ELECTRONEUTRAL NA + -COUPLED HCO 3 − TRANSPORTERS IN FRESHLY DISSOCIATED MOUSE MEDULLARY RAPHÉ NEURONS

doi: 10.1016/j.neuroscience.2013.02.064

Figure Lengend Snippet: Signal intensities from fluorescent markers (in arbitrary units)

Article Snippet: An anti-GAD2 antibody (mouse monoclonal IgG3, clone 26H1, directed against the GAD65, the 65 kDa isoform) was purchased from SySy, Goettingen, Germany.

Techniques: Standard Deviation

Journal: Neuroscience

Article Title: IMMUNOCYTOCHEMICAL IDENTIFICATION OF ELECTRONEUTRAL NA + -COUPLED HCO 3 − TRANSPORTERS IN FRESHLY DISSOCIATED MOUSE MEDULLARY RAPHÉ NEURONS

doi: 10.1016/j.neuroscience.2013.02.064

Figure Lengend Snippet: Co-localization of TPH antibody with EYFP-tagged neurons in the MR. (A) The DAPI marker (blue) identifies the nucleus of the cell in the first panel. The ePet-EYFP tag (yellow) identifies a serotonergic neuron in the second panel. In the third panel, the IgG3 monoclonal TPH antibody, together with the Cyanine-5-tagged anti-IgG3 secondary antibody (magenta), identifies the cell as a serotonergic neuron. In the fourth panel, the merge is an assembly of all of the images. (B) Frequency distribution of the TPH antibody (magenta histogram) for the TPH cell in panel A, which we consider a 5HT-positive neuron. The mean pixel intensity is 1236 (a.u.). (C) The DAPI marker identifies the nucleus, the EYFP tag is negative, the TPH antibody is negative, and the merge is an assembly of all of the images. (D) The frequency distribution of the TPH antibody (black histogram) for the TPH cell in panel C, which we consider a 5HT-negative cell. The mean pixel intensity is 178 (a.u.). (E) Summary of the total counts of cells: 105 DAPI+, 34 EYFP+/TPH+, 71 EYFP−/TPH−. (F) Mean frequency distribution of the TPH signal (magenta histogram) in 34 EYFP+/TPH+ cells. The mean intensity is 1480 (a.u.). Mean frequency distribution of the TPH signal (black histogram) in 71 EYFP−/TPH− cells. The mean pixel intensity is 477 (a.u.). (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)

Article Snippet: An anti-GAD2 antibody (mouse monoclonal IgG3, clone 26H1, directed against the GAD65, the 65 kDa isoform) was purchased from SySy, Goettingen, Germany.

Techniques: Marker

Journal: Neuroscience

Article Title: IMMUNOCYTOCHEMICAL IDENTIFICATION OF ELECTRONEUTRAL NA + -COUPLED HCO 3 − TRANSPORTERS IN FRESHLY DISSOCIATED MOUSE MEDULLARY RAPHÉ NEURONS

doi: 10.1016/j.neuroscience.2013.02.064

Figure Lengend Snippet: Mutual exclusivity of GAD2 antibody and EYFP-tagged neurons in the MR. (A) GABAergic neuron. The GAD2 antibody (cyan) identifies a GABAergic neuron, whereas the EYFP tag (yellow) is negative. (B) The mean frequency distribution of the GAD2 signal in 5 GAD2-positive cells (cyan histogram, left panel). Of 5 GAD2-positive cells, 0 were EYFP positive. The mean intensity is 689 (a.u.). (C) The mean frequency distribution of the GAD2 signal in 20 GAD2-negative cells (black histogram, right panel). The mean pixel intensity is 220 (a.u.). (D) Serotonergic neuron. (D) The GAD2 antibody does not label the cell, but the EYFP tag is positive. Of the 7 EYFP-positive cells, 0 were GAD-positive. (E) The mean frequency distribution of the GAD2 signal in 7 EYFP-positive cells. The mean pixel intensity is 253 (a.u.). (F) Summary of total counts of cells: 25 DAPI+, 5 EYFP−/GAD2+, and 7 EYFP+/GAD2−. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)

Article Snippet: An anti-GAD2 antibody (mouse monoclonal IgG3, clone 26H1, directed against the GAD65, the 65 kDa isoform) was purchased from SySy, Goettingen, Germany.

Techniques:

Journal: Neuroscience

Article Title: IMMUNOCYTOCHEMICAL IDENTIFICATION OF ELECTRONEUTRAL NA + -COUPLED HCO 3 − TRANSPORTERS IN FRESHLY DISSOCIATED MOUSE MEDULLARY RAPHÉ NEURONS

doi: 10.1016/j.neuroscience.2013.02.064

Figure Lengend Snippet: Individual labeling of SLC4A transporters, using rabbit polyclonal antibodies. (A) The IgG polyclonal NBCn1 antibody—together with the horseradish peroxidase (HRP)-tagged IgG antibody, followed by signal amplification using the Tetramethylrhodamine (TMR) System (red)—identifies NBCn1 in a cell from a WT mouse (upper left panel). In cells from an NBCn1-KO mouse, we detect only a very faint signal (lower left panel). The two right panels show the corresponding DIC images. (B) Representative z-sections (for details, see legend of Fig. 1) for cell on left side of panel A (xy sections, 1230 nm). (C) The IgG polyclonal NDCBE antibody likewise identifies NDCBE in a cell from a WT mouse (upper left panel). We detect only a very faint signal from a cell from an NDCBE-KO mouse (lower left panel). The two right panels show the DIC images. (D) Representative z-sections for cell on the left side of panel C (xy sections, 360 nm). (E) The IgG polyclonal NBCn2 antibody likewise recognizes NBCn2 in a cell from a WT mouse (upper left panel). In cells from an NBCn2-KO mouse, the signal is extremely faint (lower left panel). The two right panels show the DIC images. (F) Representative z-sections for cell on left side of panel E (xy sections, 410 nm). Scale bar = 10 μm. Images were acquired with a 60× objective. These panels show that the NBCn1, NDCBE, and NBCn2 antibodies are within the cytoplasm, and presumably the cell membrane, but excluded from the nucleus. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)

Article Snippet: An anti-GAD2 antibody (mouse monoclonal IgG3, clone 26H1, directed against the GAD65, the 65 kDa isoform) was purchased from SySy, Goettingen, Germany.

Techniques: Labeling, Amplification

Journal: Neuroscience

Article Title: IMMUNOCYTOCHEMICAL IDENTIFICATION OF ELECTRONEUTRAL NA + -COUPLED HCO 3 − TRANSPORTERS IN FRESHLY DISSOCIATED MOUSE MEDULLARY RAPHÉ NEURONS

doi: 10.1016/j.neuroscience.2013.02.064

Figure Lengend Snippet: Co-labeling studies of serotonergic neurons with nNCBT antibodies. (A) NBCn1. The DAPI marker (blue) identifies the nucleus within a cell (see Fig. 3). The IgG1 mouse monoclonal MAP2 antibody (green) identifies the cell as a neuron (see Fig. 1). The IgG3 mouse monoclonal TPH antibody (magenta) identifies the neuron as a serotonergic neuron (see Fig. 1 and Fig. 3). The IgG rabbit polyclonal antibody (red) identifies NBCn1 within the neuron (Fig. 5). The merge (white) is an assembly of all panels except DAPI. Thus, the cell is a serotonergic neuron that contains the NBCn1 transporter (B) NDCBE. The cell stains positive for DAPI, MAP2, TPH, and NDCBE. (C) NBCn2. The cell stains positive for DAPI, MAP2, TPH, and NBCn2. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)

Article Snippet: An anti-GAD2 antibody (mouse monoclonal IgG3, clone 26H1, directed against the GAD65, the 65 kDa isoform) was purchased from SySy, Goettingen, Germany.

Techniques: Labeling, Marker

Journal: Neuroscience

Article Title: IMMUNOCYTOCHEMICAL IDENTIFICATION OF ELECTRONEUTRAL NA + -COUPLED HCO 3 − TRANSPORTERS IN FRESHLY DISSOCIATED MOUSE MEDULLARY RAPHÉ NEURONS

doi: 10.1016/j.neuroscience.2013.02.064

Figure Lengend Snippet: Frequency distribution of neuronal subtypes and nNCBTs

Article Snippet: An anti-GAD2 antibody (mouse monoclonal IgG3, clone 26H1, directed against the GAD65, the 65 kDa isoform) was purchased from SySy, Goettingen, Germany.

Techniques: Cell Counting

Journal: Neuroscience

Article Title: IMMUNOCYTOCHEMICAL IDENTIFICATION OF ELECTRONEUTRAL NA + -COUPLED HCO 3 − TRANSPORTERS IN FRESHLY DISSOCIATED MOUSE MEDULLARY RAPHÉ NEURONS

doi: 10.1016/j.neuroscience.2013.02.064

Figure Lengend Snippet: Summary of data on the co-labeling of nNCBTs and GAD2. The data summarized here come from many experiments like that in Fig. 9. (A) Summary of the total counts of cells in NBCn1 study. The legend beneath the bars indicates whether the cells were positive (+), negative (−), or either (blank cell) for MAP2, GAD2, and NBCn1. Thus, the left-most group of three bars indicates that we analyzed 40 MAP2+ cells, two of which were NBCn1−, and 38 of which were NBCn1+. (B) The scatter diagram describes the relationship between the intensities of the NBCn1 signal and GAD2 signal. (C) Summary of the total counts of cells in NDCBE study. (D) The scatter diagram describes the relationship between the intensities of the NDCBE signal and GAD2 signal. (E) Summary of the total counts of cells in NBCn2 study. (F) The scatter diagram describes the relationship between the intensities of the NBCn2 signal and GAD2 signal. Based on simple linear regression, the coefficient of determination R2 was <0.3 in panels B, D, and F.

Article Snippet: An anti-GAD2 antibody (mouse monoclonal IgG3, clone 26H1, directed against the GAD65, the 65 kDa isoform) was purchased from SySy, Goettingen, Germany.

Techniques: Labeling

Journal: Neuroscience

Article Title: IMMUNOCYTOCHEMICAL IDENTIFICATION OF ELECTRONEUTRAL NA + -COUPLED HCO 3 − TRANSPORTERS IN FRESHLY DISSOCIATED MOUSE MEDULLARY RAPHÉ NEURONS

doi: 10.1016/j.neuroscience.2013.02.064

Figure Lengend Snippet: Co-labeling studies of GABAergic neurons with nNCBT antibodies. (A) NBCn1. DAPI (blue) identifies the nucleus within a cell (see Fig. 3). The IgG1 mouse monoclonal MAP2 antibody (green) identifies the cell as a neuron (see Fig. 1). The IgG3 mouse monoclonal GAD2 antibody (cyan) identifies the neuron as a GABAergic neuron (see Figs. 1 and ​and4).4). The IgG rabbit polyclonal antibody (red) identifies NBCn1 within the neuron. The merge (white) is an assembly of all panels except DAPI. Thus, the cell is a GABAergic neuron that contains NBCn1. (B) NDCBE. The cell is positive for DAPI, MAP2, GAD2, and NDCBE. (C) NBCn2. The cell is positive for DAPI, MAP2, GAD2, and NBCn2. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)

Article Snippet: An anti-GAD2 antibody (mouse monoclonal IgG3, clone 26H1, directed against the GAD65, the 65 kDa isoform) was purchased from SySy, Goettingen, Germany.

Techniques: Labeling

Journal: Neuroscience

Article Title: IMMUNOCYTOCHEMICAL IDENTIFICATION OF ELECTRONEUTRAL NA + -COUPLED HCO 3 − TRANSPORTERS IN FRESHLY DISSOCIATED MOUSE MEDULLARY RAPHÉ NEURONS

doi: 10.1016/j.neuroscience.2013.02.064

Figure Lengend Snippet: Overall frequency distribution of neuronal populations

Article Snippet: An anti-GAD2 antibody (mouse monoclonal IgG3, clone 26H1, directed against the GAD65, the 65 kDa isoform) was purchased from SySy, Goettingen, Germany.

Techniques:

Journal: Neuroscience

Article Title: IMMUNOCYTOCHEMICAL IDENTIFICATION OF ELECTRONEUTRAL NA + -COUPLED HCO 3 − TRANSPORTERS IN FRESHLY DISSOCIATED MOUSE MEDULLARY RAPHÉ NEURONS

doi: 10.1016/j.neuroscience.2013.02.064

Figure Lengend Snippet: Frequency distribution of nNCBTs among TPH-positive and GAD2-positive neurons

Article Snippet: An anti-GAD2 antibody (mouse monoclonal IgG3, clone 26H1, directed against the GAD65, the 65 kDa isoform) was purchased from SySy, Goettingen, Germany.

Techniques:

Journal: Neuroscience

Article Title: IMMUNOCYTOCHEMICAL IDENTIFICATION OF ELECTRONEUTRAL NA + -COUPLED HCO 3 − TRANSPORTERS IN FRESHLY DISSOCIATED MOUSE MEDULLARY RAPHÉ NEURONS

doi: 10.1016/j.neuroscience.2013.02.064

Figure Lengend Snippet: Identification of neuronal cell types in the MR, using mouse monoclonal antibodies. (A) The IgG1 monoclonal MAP2 antibody, together with the Cyanine-2-tagged anti-IgG1 secondary antibody (green), identifies a neuron (upper left panel) that is also shown in a DIC image (upper right). In the absence of the primary antibody, the secondary antibody did not recognize any cells, one example of which is shown here as a negative control (lower left). The DIC image shows the cell at this location (lower right). (B) Representative z-sections for neuron on left side of panel A (details below). (C) The IgG3 monoclonal TPH antibody, together with the Cyanine-5-tagged anti-IgG3 secondary antibody (magenta), identifies a serotonergic neuron (upper left panel). The staining of the secondary antibody is virtually eliminated in the absence of the primary antibody (lower left panel). The two right panels show the DIC images. (D) Representative z-sections for neuron on left side of panel C (details below). (E) IgG3 monoclonal GAD2 antibody, together with the Cyanine-5-tagged anti-IgG3 secondary antibody (cyan), identifies a GABAergic neuron (upper left panel). The staining of the secondary antibody is virtually eliminated in the absence of the primary antibody (lower left panel). The two right panels show the DIC images. Details on z-sections (panels B, D, F): For each, the main picture is an xy section (MAP2 500 nm/slice; TPH 60 nm/slice; GAD2 235 nm/slice) per slice. The yellow cross-hairs indicate the planes of the two side views: the xz section (shown below the main picture) and the yz section (shown to the right of the main picture). In the xz and yz sections, the green lines show the plane of the xy section. Scale bar = 10 μm. Images were acquired with a 60× objective. These panels show that the MAP2, TPH, and GAD2 antibodies are within the cytoplasm but excluded from the nucleus. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)

Article Snippet: An anti-GAD2 antibody (mouse monoclonal IgG3, clone 26H1, directed against the GAD65, the 65 kDa isoform) was purchased from SySy, Goettingen, Germany.

Techniques: Bioprocessing, Negative Control, Staining

Journal: Neuroscience

Article Title: IMMUNOCYTOCHEMICAL IDENTIFICATION OF ELECTRONEUTRAL NA + -COUPLED HCO 3 − TRANSPORTERS IN FRESHLY DISSOCIATED MOUSE MEDULLARY RAPHÉ NEURONS

doi: 10.1016/j.neuroscience.2013.02.064

Figure Lengend Snippet: Frequency distributions of the individual cell types. The data summarized here come from many images like that in Fig. 1. (A) The mean frequency distribution (green histogram) of 10 MAP2-positive cells describes the average number of pixels for each pixel intensity. The mean intensity of cells in the MAP2 population was 876 (arbitrary units, a.u.). (B) The frequency distribution of the negative controls (black histogram) is the average of 10 cells. The average mean intensity from negative controls was 177 (a.u.). The green data represent a re-plot of the data from panel A. (C) The mean frequency distribution (Cyan histogram) of 13 GAD2-positive cells yields a mean intensity of 1175 (a.u.). (D) The frequency distribution of the negative controls (black histogram) is an average of 20 cells. The average mean intensity from negative controls was 220 (a.u.). The cyan data represent a re-plot of the data from panel C. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)

Article Snippet: An anti-GAD2 antibody (mouse monoclonal IgG3, clone 26H1, directed against the GAD65, the 65 kDa isoform) was purchased from SySy, Goettingen, Germany.

Techniques:

Journal: Neuroscience

Article Title: IMMUNOCYTOCHEMICAL IDENTIFICATION OF ELECTRONEUTRAL NA + -COUPLED HCO 3 − TRANSPORTERS IN FRESHLY DISSOCIATED MOUSE MEDULLARY RAPHÉ NEURONS

doi: 10.1016/j.neuroscience.2013.02.064

Figure Lengend Snippet: Signal intensities from fluorescent markers (in arbitrary units)

Article Snippet: An anti-GAD2 antibody (mouse monoclonal IgG3, clone 26H1, directed against the GAD65, the 65 kDa isoform) was purchased from SySy, Goettingen, Germany.

Techniques: Standard Deviation

Journal: Neuroscience

Article Title: IMMUNOCYTOCHEMICAL IDENTIFICATION OF ELECTRONEUTRAL NA + -COUPLED HCO 3 − TRANSPORTERS IN FRESHLY DISSOCIATED MOUSE MEDULLARY RAPHÉ NEURONS

doi: 10.1016/j.neuroscience.2013.02.064

Figure Lengend Snippet: Mutual exclusivity of GAD2 antibody and EYFP-tagged neurons in the MR. (A) GABAergic neuron. The GAD2 antibody (cyan) identifies a GABAergic neuron, whereas the EYFP tag (yellow) is negative. (B) The mean frequency distribution of the GAD2 signal in 5 GAD2-positive cells (cyan histogram, left panel). Of 5 GAD2-positive cells, 0 were EYFP positive. The mean intensity is 689 (a.u.). (C) The mean frequency distribution of the GAD2 signal in 20 GAD2-negative cells (black histogram, right panel). The mean pixel intensity is 220 (a.u.). (D) Serotonergic neuron. (D) The GAD2 antibody does not label the cell, but the EYFP tag is positive. Of the 7 EYFP-positive cells, 0 were GAD-positive. (E) The mean frequency distribution of the GAD2 signal in 7 EYFP-positive cells. The mean pixel intensity is 253 (a.u.). (F) Summary of total counts of cells: 25 DAPI+, 5 EYFP−/GAD2+, and 7 EYFP+/GAD2−. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)

Article Snippet: An anti-GAD2 antibody (mouse monoclonal IgG3, clone 26H1, directed against the GAD65, the 65 kDa isoform) was purchased from SySy, Goettingen, Germany.

Techniques:

Journal: Neuroscience

Article Title: IMMUNOCYTOCHEMICAL IDENTIFICATION OF ELECTRONEUTRAL NA + -COUPLED HCO 3 − TRANSPORTERS IN FRESHLY DISSOCIATED MOUSE MEDULLARY RAPHÉ NEURONS

doi: 10.1016/j.neuroscience.2013.02.064

Figure Lengend Snippet: Frequency distribution of neuronal subtypes and nNCBTs

Article Snippet: An anti-GAD2 antibody (mouse monoclonal IgG3, clone 26H1, directed against the GAD65, the 65 kDa isoform) was purchased from SySy, Goettingen, Germany.

Techniques: Cell Counting

Journal: Neuroscience

Article Title: IMMUNOCYTOCHEMICAL IDENTIFICATION OF ELECTRONEUTRAL NA + -COUPLED HCO 3 − TRANSPORTERS IN FRESHLY DISSOCIATED MOUSE MEDULLARY RAPHÉ NEURONS

doi: 10.1016/j.neuroscience.2013.02.064

Figure Lengend Snippet: Summary of data on the co-labeling of nNCBTs and GAD2. The data summarized here come from many experiments like that in Fig. 9. (A) Summary of the total counts of cells in NBCn1 study. The legend beneath the bars indicates whether the cells were positive (+), negative (−), or either (blank cell) for MAP2, GAD2, and NBCn1. Thus, the left-most group of three bars indicates that we analyzed 40 MAP2+ cells, two of which were NBCn1−, and 38 of which were NBCn1+. (B) The scatter diagram describes the relationship between the intensities of the NBCn1 signal and GAD2 signal. (C) Summary of the total counts of cells in NDCBE study. (D) The scatter diagram describes the relationship between the intensities of the NDCBE signal and GAD2 signal. (E) Summary of the total counts of cells in NBCn2 study. (F) The scatter diagram describes the relationship between the intensities of the NBCn2 signal and GAD2 signal. Based on simple linear regression, the coefficient of determination R2 was <0.3 in panels B, D, and F.

Article Snippet: An anti-GAD2 antibody (mouse monoclonal IgG3, clone 26H1, directed against the GAD65, the 65 kDa isoform) was purchased from SySy, Goettingen, Germany.

Techniques: Labeling

Journal: Neuroscience

Article Title: IMMUNOCYTOCHEMICAL IDENTIFICATION OF ELECTRONEUTRAL NA + -COUPLED HCO 3 − TRANSPORTERS IN FRESHLY DISSOCIATED MOUSE MEDULLARY RAPHÉ NEURONS

doi: 10.1016/j.neuroscience.2013.02.064

Figure Lengend Snippet: Co-labeling studies of GABAergic neurons with nNCBT antibodies. (A) NBCn1. DAPI (blue) identifies the nucleus within a cell (see Fig. 3). The IgG1 mouse monoclonal MAP2 antibody (green) identifies the cell as a neuron (see Fig. 1). The IgG3 mouse monoclonal GAD2 antibody (cyan) identifies the neuron as a GABAergic neuron (see Figs. 1 and ​and4).4). The IgG rabbit polyclonal antibody (red) identifies NBCn1 within the neuron. The merge (white) is an assembly of all panels except DAPI. Thus, the cell is a GABAergic neuron that contains NBCn1. (B) NDCBE. The cell is positive for DAPI, MAP2, GAD2, and NDCBE. (C) NBCn2. The cell is positive for DAPI, MAP2, GAD2, and NBCn2. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)

Article Snippet: An anti-GAD2 antibody (mouse monoclonal IgG3, clone 26H1, directed against the GAD65, the 65 kDa isoform) was purchased from SySy, Goettingen, Germany.

Techniques: Labeling

Journal: Neuroscience

Article Title: IMMUNOCYTOCHEMICAL IDENTIFICATION OF ELECTRONEUTRAL NA + -COUPLED HCO 3 − TRANSPORTERS IN FRESHLY DISSOCIATED MOUSE MEDULLARY RAPHÉ NEURONS

doi: 10.1016/j.neuroscience.2013.02.064

Figure Lengend Snippet: Overall frequency distribution of neuronal populations

Article Snippet: An anti-GAD2 antibody (mouse monoclonal IgG3, clone 26H1, directed against the GAD65, the 65 kDa isoform) was purchased from SySy, Goettingen, Germany.

Techniques:

Journal: Neuroscience

Article Title: IMMUNOCYTOCHEMICAL IDENTIFICATION OF ELECTRONEUTRAL NA + -COUPLED HCO 3 − TRANSPORTERS IN FRESHLY DISSOCIATED MOUSE MEDULLARY RAPHÉ NEURONS

doi: 10.1016/j.neuroscience.2013.02.064

Figure Lengend Snippet: Frequency distribution of nNCBTs among TPH-positive and GAD2-positive neurons

Article Snippet: An anti-GAD2 antibody (mouse monoclonal IgG3, clone 26H1, directed against the GAD65, the 65 kDa isoform) was purchased from SySy, Goettingen, Germany.

Techniques: